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MOLECULAR BIOLOGY LABORATORY

In our department, a routine laboratory, which can reflect the concept of molecular medicine and gained technological competence in the field of genomics, serves clinics. Molecular Diagnosis services provided by the department routinely are listed below. Molecular Biology laboratory has been performing more than 80 routine molecular analyses in many clinics since 2004 and still provides routine molecular analysis service with Ege University Genetic Diagnosis Center. Our vision in our department is to carry out basic and applied researches and develop laboratory services at the gene and cell levels, especially for new diagnosis and treatment methods. In our department, mutation/polymorphism analysis and genotyping are performed in these disease groups to evaluate the susceptibility to diseases such as thrombophilia, hypertension, and hyperlipidemia, which are common in society. The tests performed are shown in the table below. Also, to help leukemia diseases in terms of molecular diagnosis, different translocation tests are performed in the leukemia panel and B-Cell Immunoglobulin gene clonality and T-cell receptor gene clonality tests are carried out to help diagnose and classify lymphoma. A new generation of DNA sequencing (NGS) for drug targeting is also performed for various cancers.

Molecular Analyisi in Molecular Biplogy Laboratory

 

Leukemia Panel 

t(1;11)(p32;q23) MLL-EPS15

t(9;11)(p22;q23) MLL-MLLT3

t(12;21)(p13;q22) ETV6-RUNX1

t(1;11)(q21;q23) MLL-MLLT11

t(9;12)(q34;p13) ETV6-ABL1

t(12;22)(p13;q11-12) ETV6-MN1

t(1;19)(q23;p13) TCF3-PBX1

t(9;22)(q34;q11) BCR-ABL1 (m-bcr, p190)

t(15;17)(q24;q21) PML-RARA (bcr1, L)

t(3;21)(q26;q22) RUNX1-MECOM

t(9;22)(q34;q11) BCR-ABL1 (M-bcr, p210)

t(15;17)(q24;q21) PML-RARA (bcr2, V)

t(3;5)(q25.1;q35) NPM1-MLF1

t(9;22)(q34;q11) BCR-ABL1 (µ-bcr, p230)

t(15;17)(q24;q21) PML-RARA (bcr3, S)

t(4;11)(q21;q23) MLL-AFF1

t(9;9)(q34;q34) SET-NUP214

t(16;21)(p11;q22) FUS-ERG

t(5;12)(q33;p13) ETV6-PDGFRB

t(10;11)(p12;q23) MLL-MLLT10

inv(16)(p13;q22) CBFB-MYH11

t(5;17)(q35;q21) NPM1-RARA

t(11;17)(q23;q21) MLL-MLLT6

t(17;19)(q22;p13) TCF3-HLF

t(6;11)(q27;q23) MLL-MLLT4

t(11;17)(q23;q21) ZBTB16-RARA

t(X;11)(q13;q23) MLL-FOXO4

t(6;9)(p23;q34) DEK-NUP214

t(11;19)(q23;p13.1) MLL-ELL

 

 

NGS Cancer Panel

AKT1

ALK

BRAF

CTNNB1

DDR2

EGFR

ERBB2

ERBB3

ERBB4

ESR1

FBXW7

FGFR1

FGFR2

FGFR3

FLT3

GNA11

GNAQ

HRAS

KIT

KRAS

MAP2K1

MAP2K2

MET

NOTCH1

NRAS

PDGFRA

PIK3CA

RAF1

SMAD4

STK11

 

NGS Breast Cancer Panel

BRCA1

BRCA2

PTEN

TP53

 

 

NGS Lung Cancer Panel

AKT1

ALK

BRAF

DDR2

EGFR

ERBB2/HER2

ESR1

FGFR1

KIT

KRAS

MAP2K1

MET

NRAS

NTRK1

PDGFRA

PIK3CA

PTEN

RICTOR

ROS1

 

 

NGS Lung Cancer Fussion Panel

AGTRAP-BRAF

AKAP9-BRAF

ATIC-ALK

CCDC6-RET

CD74-NRG1

CD74-NTRK1

CD74-ROS1

CEP89-BRAF

CLCN6-BRAF

CLTC-ALK

DCTN1-ALK

EML4-ALK

ERC1-RET

ERC1-ROS1

ESRP1-RAF1

EZR-ROS1

FAM131B-BRAF

FCHSD1-BRAF

FGFR1-PLAG1

FGFR1-TACC1

FGFR1-ZNF703

FGFR3-TACC3

FN1-ALK

GATM-BRAF

GNAI1-BRAF

GOLGA5-RET

GOPC-ROS1

HACL1-RAF1

HERPUD1-BRAF

HIP1-ALK

HOOK3-RET

KIF5B-ALK

KIF5B-RET

KLC1-ALK

LMNA-NTRK1

LRIG3-ROS1

LSM14A-BRAF

MET exon 14 skipping

MKRN1-BRAF

MYO5A-ROS1

NCOA4-RET

NPM1-ALK

NTRK1-TPM3

PCM1-RET

PPFIBP1-ALK

PPFIBP1-ROS1

PRKAR1A-RET

PWWP2A-ROS1

RAF1-DAZL

RANBP2-ALK

RNF130-BRAF

SDC4-ROS1

SEC31A-ALK

SLC34A2-ROS1

SLC45A3-BRAF

SQSTM1-ALK

STRN-ALK

TFG-ALK

TFG-NTRK1

TPM3-ALK

TPM3-NTRK1

TPM3-ROS1

TPM4-ALK

TRIM24-RET

TRIM33-RET

UBE2L3-KRAS

VCL-ALK

ZSCAN30-BRAF


			

 

 

 

Cell Culture Laboratory 

Bu laboratuvarımızda, fibroblast kültürü, beyin tümörü hücre kültürleri ile çeşitli hücre serilerinin kültürleri yapılabilmektedir.


Ege Üniversitesi

EGE UNIVERSITY